Structural and conformational analysis of immunoglobulin-derived N-linked oligosaccharides.

To whom correspondence should be addressed. Despite a number of studies extending over the last 10 years the exact number of IgGt oligosaccharide chains, oligosaccharide type and sequence remains controversial (Abel el al., 1968). In addition, rigorous studies of species, allotype and sub-class related differences have not yet been performed. Oligosaccharide units have been reported to be present on the light chain (variable region) and heavy chain (Fd, hinge and C,2 domain) (Spiegelberg et al., 1970). The oligosaccharides attached to the C,2 domain have been conserved throughout evolution and are the focus of this report. The multiplicity and variability of glycosylation on the Fab, t Abbreviations used: IgG, immunoglobulin G; NOE, nuclear Overhauser enhancement.